THE MOLECULAR WEIGHT OF CRYSTALLINE CATALASE
نویسندگان
چکیده
منابع مشابه
The Molecular Weight of Crystalline Catalase.
In 1933 Stern (2) used the diffusion method of Northrop and Anson (3) to determine the molecular weights of purified catalase from horse liver, pig liver, cow liver, horse kidney, and cow erythrocytes. He found the average value for the molecular weight of catalase to be 68,900, and stated that the catalase molecule has a dimension similar to that of hemoglobin. In 1938 Stern and Wyckoff (4) pu...
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Electrophoretic studies on purified crystalline ribonuclease showed the absence of any impurities differing in mobility from the bulk of material. The isoelectric point of ribonuclease was found by electrophoresis to be at about pH 7.8. Ultracentrifuge studies indicated fair homogeneity of ribonuclease in solution. Only one moving component has been observed. The molecular weight of ribonucleas...
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The recent work of Fishman, Wilhelmi, and Russell (1,2) has made available a crystalline growth hormone isolated from the bovine anterior pituitary gland. It was of considerable interest to determine the sedimentation behavior of this protein in the ultracentrifuge and, at the same time, to obtain the molecular weight by this method. The amorphous preparation of Li, Evans, and Simpson (3) has n...
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The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
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The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1938
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)73977-x